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You are here:Home » Antibodies » Abs to Lectins » Anti -Arachis hypogaea (PNA, Peanut Agglutinin) (Texas Red)

Anti -Arachis hypogaea (PNA, Peanut Agglutinin) (Texas Red)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified IH IC IF
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity. T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies.
Catalog #A3305-39
ApplicationsSuitable for use in Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Carbohydrate Specificity Terminal b-Galactose
Inhibitory Carbohydrate Lactose > b-Galactose
Activity 1ug/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.
Texas Red /Protein Ratio (OD595/OD280) 2.4
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Texas Red® conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M Sodium Bicarbonate, pH 9.0-9.5. Labeled with Texas red.
PurityPurified by gel filtration.
ImmunogenArachis hypogaea (PNA, peanut).
SpecificityRecognizes Arachis hypogaea (PNA, peanut).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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