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Anti -Arc p34 (ARP2/3 Complex 34kD subunit, Actin-related Protein 2/3 Complex subunit 2)
Anti -Arc p34 (ARP2/3 Complex 34kD subunit, Actin-related Protein 2/3 Complex subunit 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IC |
|
| Catalog # | A3327 | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml detects p34-ARC in RIPA lysates from human A431, Jurkat and mouse 3T3 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p34-Arc (1ug/ml). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. | | Immunohistochemistry: 10ug/ml detects p34-Arc in mouse array tissue and formalin-fixed, paraffin-embedded human adenocarcinoma tissue. | | Immunocytochemistry: Can be detected in C2C12 cells. | | Optimal dilution determined by the researcher. | | Included Positive Antigen Control | A0001-50: A431 Cell Lysate, Non-Stimulated | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | N/A | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa285- 298 (C-EKKEMKTITGKTFS) of human containing a N-terminal cysteine (KLH coupled). | | Specificity | Recognizes ARC p34, Mr 34kD of the Arp2/3 complex. Species Crossreactivity: Human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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