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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -ARFBP1 (HUWE1. HECT, UBA and WWE domain containing 1, ARF-binding protein 1, ARF-BP1, E3 ubiquitin-protein ligase HUWE1, HECT, UBA and WWE domain-containing protein 1, HectH9, HECTH9, Homologous to E6AP carboxyl terminus homologous protein 9, HSPC272, Ib7

Anti -ARFBP1 (HUWE1. HECT, UBA and WWE domain containing 1, ARF-binding protein 1,
ARF-BP1, E3 ubiquitin-protein ligase HUWE1, HECT, UBA and WWE domain-containing protein 1,
HectH9, HECTH9, Homologous to E6AP carboxyl terminus homologous protein 9, HSPC272, Ib7

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IC
The ARF tumor suppressor is a critical regulator of p53 stability (reviewed in 1). In addition to p53, ARF1 binds to other proteins such as MDM2 (2) and ARF-BP1, a large protein containing HECT, UBA and WWE motifs (3). ARFBP1 directly binds and ubiquitinates p53; this activity is
inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents (4).
Catalog #A3330-11
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunocytochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlDaudi cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
Immunogen19 amino acid peptide from near the carboxy terminus of human
ARF-BP1 (GenBank accession no. NP_113584).
SpecificityRecognizes human ARF-BP1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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