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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1, GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein Ligase TRIM23, RING Finger Protein 46, RNF46)

Anti -ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1,
GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein
Ligase TRIM23, RING Finger Protein 46, RNF46)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IC
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARFBP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Catalog #A3330-11A
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunocytochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlDaudi cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to 19aa from near the C-terminus of human ARF-BP1.
SpecificityRecognizes human ARF-BP1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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