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Antibodies
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Abs to GTPase, Rab, Ras, Rho Proteins
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Anti -ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1, GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein Ligase TRIM23, RING Finger Protein 46, RNF46)
Anti -ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1,
GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein
Ligase TRIM23, RING Finger Protein 46, RNF46)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E B IC |
|
| The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARFBP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents. | | | Catalog # | A3330-11A | | Applications | Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml | | Immunocytochemistry: 5ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | Daudi cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.02% sodium azide. | | Purity | Purified | | Immunogen | Synthetic peptide corresponding to 19aa from near the C-terminus of human ARF-BP1. | | Specificity | Recognizes human ARF-BP1. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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