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You are here:Home » Antibodies » Abs to Enzymes » Anti -ARG1 (Arginase-1, Liver-type Arginase, Type I Arginase)

Anti -ARG1 (Arginase-1, Liver-type Arginase, Type I Arginase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.
Catalog #A3340-14A3
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsPeptide ELISA: 1:2000
Western Blot: 0.03-0.1ug/ml. Band of ~35kD was observed in human liver lysates. Additional bands of unknown identity were also consistently observed at 19-22kD. These bands were successfully blocked by incubation with the immunizing peptide.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Liver lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-REGNHKPIDYLNPPK from the C-terminus of human ARG1 (NP_000036.2).
SpecificityRecognizes human ARG1. Species sequence homology: canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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