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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -Arg, SH3/SH2 (Tyrosine Kinase Abl-related Gene)

Anti -Arg, SH3/SH2 (Tyrosine Kinase Abl-related Gene)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Catalog #A3340-35
ApplicationsSuitable for use in Western Blot., Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:1000 detects Arg in RIPA lysates from mouse brain cytosol. Mouse brain cytosol was resolved by electrophoresis, transferred to nitrocellulose and probed with A3340-35. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: Reported to detect Arg, in either paraf n-embedded or frozen murine brain sections.
Immunoprecipitation: Reported to immunoprecipitate enzymatically active Arg from 100ug mouse brain RIPA lysates.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot Determined
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by immunoaffinity chromatography
ImmunogenGST fusion protein corresponding to the SH2 and SH3 domains of murine Arg (Abl-related gene), residues 112-259. Species Sequence Homology: human and Xenopus .
SpecificityRecognizes murine Arg, doublet, Mr 135 and 140kD. Two non-speci c proteins were also detected at ~Mr 170 and 250kD
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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