| Catalog # | A3340-35 |
| Applications | Suitable for use in Western Blot., Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500-1:1000 |
| Western Blot Analysis: 1:500 detects Arg in RIPA lysates from mouse brain cytosol. Mouse brain cytosol was resolved by electrophoresis, transferred to nitrocellulose and probed with A3340-35 (1:500). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunohistochemistry: Reported to detect Arg, in either paraf n-embedded or frozen murine brain sections. |
| Immunoprecipitation: Reported to immunoprecipitate enzymatically active Arg from 100ug mouse brain RIPA lysates. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | Not determined. |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by affinity chromatography. |
| Immunogen | GST fusion protein corresponding to the SH2 and SH3 domains of murine Arg (Abl-related gene), residues 112–259. |
| Specificity | Recognizes murine Arg, doublet, Mr 135 and 140kD. Two non-speci c proteins were also detected at ~Mr 170 and 250kD Species sequence homology: human and Xenopus. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
