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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Arginine, dimethyl, Antibody Sampler Kit, BioAssay™

Arginine, dimethyl, Antibody Sampler Kit, BioAssay™

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Specifications

Catalog #A3550-06
Kit ComponentsA3550-06A Arginine, symmetric, dimethyl (SYM10) 1x25ul (Human, mouse) (WB, IP, ICC)
Serum
Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G (which R* denotes symmetric dimethylation of arginine) (KLH).
A3550-06B Arginine, symmetric, dimethyl (SYM11) 1x25ug (Human) (WB) Purified by Protein A affinity chromatography. Supplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide), 30% glycerol. Concentration 1mg/ml
Synthetic peptide corresponding to KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) (KLH).
*When using A3550-06B as primary antibody, add 0.05% Twenn-20 to blocking buffer in steps 2. 3 and 5.
A3550-06C Arginine, symmetric, dimethyl (ASYM24) 1x25ul (Human, mouse) (WB, IP, ICC) Serum Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol. Synthetic peptide corresponding to KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G (which R* denotes asymmetric dimethylation of arginine) (KLH).
Positive Control (Included)J9000 Jurkat Cell Lysate. 1x100ul Supplied as a liquid in modified RIPA buffer diluted with non-reducing sample buffer.
Concentration: 1mg/ml
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsA3550-06A: 1:500-1:1000
A3550-06B: 1-2ug/ml
A3550-06C: 1:500-1:1000
Storage and StabilityAliquot to avoid repeated freezing and thawing. Store at -20°C. Antibody aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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