| Catalog # | A3550-06 |
| Kit Components | A3550-06A Arginine, symmetric, dimethyl (SYM10) 1x25ul (Human, mouse) (WB, IP, ICC) |
| Serum |
| Supplied as a liquid in 0.05% sodium azide, 30% glycerol. |
| Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G (which R* denotes symmetric dimethylation of arginine) (KLH). |
| A3550-06B Arginine, symmetric, dimethyl (SYM11) 1x25ug (Human) (WB) Purified by Protein A affinity chromatography. Supplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide), 30% glycerol. Concentration | 1mg/ml |
| Synthetic peptide corresponding to KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) (KLH). |
| *When using A3550-06B as primary antibody, add 0.05% Twenn-20 to blocking buffer in steps 2. 3 and 5. |
| A3550-06C Arginine, symmetric, dimethyl (ASYM24) 1x25ul (Human, mouse) (WB, IP, ICC) Serum Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol. Synthetic peptide corresponding to KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G (which R* denotes asymmetric dimethylation of arginine) (KLH). |
| Positive Control (Included) | J9000 Jurkat Cell Lysate. 1x100ul Supplied as a liquid in modified RIPA buffer diluted with non-reducing sample buffer. |
| Concentration: 1mg/ml |
| Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilutions | A3550-06A: 1:500-1:1000 |
| A3550-06B: 1-2ug/ml |
| A3550-06C: 1:500-1:1000 |
| Storage and Stability | Aliquot to avoid repeated freezing and thawing. Store at -20°C. Antibody aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
