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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Arginine, symmetric, dimethyl (SYM11)

Anti -Arginine, symmetric, dimethyl (SYM11)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #A3550-07A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1-2ug/ml of this lot detected numerous proteins including SmD3 in RIPA lysates from Jurkat cells.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlJurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) (KLH).
SpecificityRecognizes SmD3 at ~16kD, and other proteins that contain arginines that are symmetrically dimethylated. Specificity of symmetrically dimethylated arginine was determined by ELISA with unmodified and symmetrically dimethylarginine containing peptides. Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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