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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Arginine, symmetric, dimethyl (SYM10)

Anti -Arginine, symmetric, dimethyl (SYM10)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IC
Catalog #A3550-07C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: A 1:500-1:1000 detects methylated p60 (unidentified protein) and Sm protein B, Bí and D in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to PVDF and probed with A3550-07C (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Detects bands at 60, 30, 32 and 16kD, corresponding to p60, Sm B, Bí and D, respectively.
Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate proteins containing symmetric dimethyl-arginine.
Immunocytochemistry: 1:500. Reported by an independent laboratory to immunostain Cajal bodies in the nuclei of HeLa, WI-38 and NIH/3T3 cells fixed with paraformaldehyde and permeabilized with triton.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlJ9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G in which (R*) denotes symmetric dimethylation of arginine). Conjugated to KLH.
SpecificityRecognizes proteins that contain multiple symmetrically dimethylated arginines, including the Sm protein B, Bí and D. Does not recognize non-methylated peptides, peptides containing asymmetrically dimethylated arginines, or a peptide containing a single symmetric dimethyl-arginine. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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