You are here:Home
»
Antibodies
»
Abs to G Proteins
»
Anti -ARHGAP1 (Rho GTPase-activating Protein 1, Rho-type GTPase-activating Protein 1, MacGAP)
Anti -ARHGAP1 (Rho GTPase-activating Protein 1, Rho-type GTPase-activating Protein 1,
MacGAP)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| ARHGAP1(also p50-RhoGAP and CDC42 GTPase-activating protein) is a 50kD member of the ARHGAP family of proteins. It is preferentially expressed in epithelium, and serves as a cytoplasmic protein that deactivates Rho-family GTPases. It does so by contributing an Arg residue (Arg282)to Cdc42 and RhoA, which activates their intrinsic GTPase activity and converts GTP-associated molecules into inactivating GDP molecules. Human ARHGAP1 is 439aa in length and contains one CRAL-TRIO domain that binds small lipophilic molecules (aa53-218), an SH3 binding site (aa228-238) and one C-terminal RhoGAP domain (aa244-431). There are phosphorylation sites at Ser51 and Tyr65. There is one potential splice form with a 24aa substitution for (aa1-150)coupled to a 26aa substitution for (aa300-439), Over (aa1-62, human ARHGAP1 shares 96% aa identity with mouse ARHGAP1. | | | Catalog # | A3570-66R | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa1-62 from human ARHGAP1 expressed in E. coli (Q07960). | | Specificity | Recognizes human, mouse and rat ARHGAP1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
