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Anti -ARK5 (NUAK Family SNF1-like Kinase 1, AMPK-related Protein Kinase 5, Omphalocele Kinase 1, KIAA0537, NUAK1, OMPHK1)
Anti -ARK5 (NUAK Family SNF1-like Kinase 1, AMPK-related Protein Kinase 5, Omphalocele
Kinase 1, KIAA0537, NUAK1, OMPHK1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| AMPK-related kinase 5, also known as NUAK1, is a 74kD intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain aa65-306 and a short isoform of the human protein is prematurely tuncated within this domain. Within aa311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5. | | | Catalog # | A3570-79C | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 12K12 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to aa311-462 from human ARK5 expressed in E. coli. | | Specificity | Recognizes human ARK5. Species Crossreactivity: Mouse and rat. Does not react with recombinant human SNARK. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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