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ARL1, Recombinant, Human (ADP-ribosylation Factor-like Protein 1)
ARL1, Recombinant, Human (ADP-ribosylation Factor-like Protein 1)
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| ADP-ribosylation factor-like protein 1(ARL1) belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. | | | Catalog # | A3570-83H | | Source | Recombinant corresponding to aa1-181 of human ARL1 protein, fused to His-tag at N-terminus, expressed in E.coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q | | Molecular Weight | 22.5kD (201aa) confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 22.5kD | | Source | E.coli | | Purity | ~95% (SDS-PAGE). | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 40% glycerol, 100mM sodium chloride, 2mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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