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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -ARL4D (ADP-ribosylation Factor-like 4D, ADP-ribosylation Factor-like Protein 4D, ADP-ribosylation Factor-like Protein 4L, ARF4L, ARL6)

Anti -ARL4D (ADP-ribosylation Factor-like 4D, ADP-ribosylation Factor-like Protein 4D,
ADP-ribosylation Factor-like Protein 4L, ARF4L, ARL6)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS).
Catalog #A3570-86D
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.3-1ug/ml
ELISA: 1:64,000
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Lung lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See A3570-86D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, C-KQDQPGALSAAEVEKR from the internal region of human ARL4D.
SpecificityRecognizes human ARL4D at ~22kD. This antibody is not expected to cross-react to other ARL4 products. Species sequence homology: mouse, canine, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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