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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -ARNT (Arylhydrocarbon Receptor Nuclear Translocator)

Anti -ARNT (Arylhydrocarbon Receptor Nuclear Translocator)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Transcriptional responses to many environmental and developmental stimuli are mediated by the basic helix-loop-helix (bHLH)-PAS family of proteins, which form homodimers that bind regulatory DNA sequences. ARNT regulates the expression of different sets of genes in response to distinct environmental challenges. ARNT forms a complex with arylhydrocarbon receptor (AHR) to activate expression of genes encoding P450. Once AHR binds it ligand, it associates with ARNT for transport into the nucleus. ARNT also dimerizes with HIF-1alpha, -2 alpha or -3 alpha in response to low oxygen concentration to induce the expression of hypoxia-regulated genes encoding vascular endothelial growth and other genes. The expression of ARNT/HIF-1beta remains fairly constant and the protein is found in the nucleus and the cytoplasm.
Catalog #A3572
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Gel Shift Assays. Other applications not tested.
Recommended DilutionsWestern Blot: 1:4000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D130
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa771-789, N771SYNNEEFPDLTMFPPFSE789 from human ARNT.
SpecificityRecognizes human ARNT at ~87kD. Species Crossreactivity: rat, mouse, monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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