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Anti -ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT Protein, Class E Basic Helix-loop-helix Protein 2, bHLHe2, Dioxin Receptor, Nuclear Translocator, Hypoxia-inducible Factor 1-beta, HIF-1-beta, HIF1-beta, BHLHE2)
Anti -ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT Protein, Class E Basic
Helix-loop-helix Protein 2, bHLHe2, Dioxin Receptor, Nuclear Translocator,
Hypoxia-inducible Factor 1-beta, HIF-1-beta, HIF1-beta, BHLHE2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH IF |
|
| Transcriptional responses to many environmental and developmental stimuli are mediated by the basic helix-loop-helix (bHLH)-PAS family of proteins, which form homodimers that bind regulatory DNA sequences. ARNT/HIF-1 beta regulates the expression of different sets of genes in response to distinct environmental challenges. ARNT forms a complex with arylhydrocarbon receptor (AHR) to activate expression of genes encoding P450. Once AHR binds it ligand, it associates with ARNT for transport into the nucleus. ARNT also dimerizes with HIF-1alpha, -2 alpha or -3 alpha in response to low oxygen concentration to induce the expression of hypoxia-regulated genes encoding vascular endothelial growth and other genes. The expression of ARNT/HIF-1beta remains fairly constant and the protein is found in the nucleus and the cytoplasm. | | | Catalog # | A3572-01B | | Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500-1:1000 | | Immunofluorescence: 10ug/ml | | Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 3D10 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant corresponding to aa1-110 from human ARNT with GST tag. MW of the GST tag alone is 26kD. | | Specificity | Recognizes human ARNT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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