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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -ARP2

Anti -ARP2

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3 and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). The ARP2/3 complex (along with N-WASP) regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).
Catalog #A3574-91
ApplicationsSuitable for use in Western Blot.
Other applications have not been tested.
Recommended DilutionsWestern blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
PuritySerum
ImmunogenSynthetic peptide (KLH-coupled) corresponding to the sequence of human ARP2.
SpecificityRecognizes endogenous levels of total human ARP2 protein. Species crossreactivity: mouse, rat, monkey, hamster and D.melanogaster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(Actin Like Protein 2, Actin-related Protein 2, ACTR2)


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