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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -ARPC2 (ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34)

Anti -ARPC2 (ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446,
p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.
Catalog #A3576
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA: >1:32,000.
Western Blot: 0.1-0.5ug/ml. ~33-34kD band observed in Hela and Human Muscle lysates (predicted MW of 34kD according to NP_005722).
Immunohistochemistry: 3-5ug/ml paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See A3576-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenC-EMKTITGKTFSSR, C Terminus
SpecificityThis antibody is expected to recognize both reported isoforms, as represented by NP_005722 and NP_690601. Species Crossreactivity: human, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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