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You are here:Home » Antibodies » Abs to Arrestin » Anti -Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

Anti -Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
Beta-arrestin 1 is a member of the arrestin family, which has been implicated in mediating the desensitization and internalization of G protein-coupled receptors (GPCR) (1). Beta-arrestin 1 is constitutively phosphorylated by Erk1/2 at serine 412 of the C-terminus and localized in the cytosol (2). When recruited to the plasma membrane after agonist stimulation of the beta2-adrenergic receptors, beta-arrestin 1 is rapidly dephosphorylated. This dephosphorylation is an essential step of beta-arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization (3).
Catalog #A3577
Applications Suitable for use in ELISA, Western Blotting and Immunoprecipitation. Other applications not tested.
Recommended Dilution Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3A14
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
PurityPurified.
ImmunogenSynthetic peptide (KLH coupled) corresponding to residues surrounding Ser412 of human beta-arrestin 1.
SpecificityDetects endogenous beta-arrestin 1 only when phosphorylated at Ser412. Does not crossreact with beta-arrestin 2. Species Crossreactivity: Mouse and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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