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You are here:Home » Antibodies » Abs to Arrestin » Anti -Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

Anti -Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Beta arrestin-1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, b-arrestin-1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs, e.g., b2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. b-arrestin-1 in the cytosol is phosphorylated by ERK1&2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras Raf ERK1&2 signaling pathway.
Catalog #A3577-01
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlPC12 and CHO-K cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to rat b-arrestin-1 that contains serine 412.
SpecificityRecognizes mouse and rat b-arrestin-1 when phosphorylated at Ser412. Species sequence homology: human and bovine (77%) b-arrestin-1 have not been tested, but are expected to react.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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