Anti -Arrestin 1,2 beta (ARRB1, ARB1, ARR1, ARRB2, ARB2, ARR2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Affinity Purified |
B IP |
|
| Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylat-ed GPCR and the eventual internalization of the receptor and | | | desensitization of GPCR signaling (1). Four distinct mam-malian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as Beta-arrestin 1) and arrestin 3 (or Beta-arrestin 2) are ubiqui-tously expressed and bind to most GPCRs (2). Beta-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). Beta arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that Beta-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10). | | | Catalog # | A3577-01H | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunoprecipitation: 1:200 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10E5 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 0.02% sodium azide and 50% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to residues surrounding Phe386 of human SGK2 protein.tissues using SGK2 antibody. | | | Specificity | Recongnizes endogenous levels of human total SGK2 protein. Species Crossreactivity: Rat, mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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