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You are here:Home » Antibodies » Abs to Arrestin » Anti -Arrestin 1,2 beta (ARRB1, ARB1, ARR1, ARRB2, ARB2, ARR2)

Anti -Arrestin 1,2 beta (ARRB1, ARB1, ARR1, ARRB2, ARB2, ARR2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B IP
Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylat-ed GPCR and the eventual internalization of the receptor and
desensitization of GPCR signaling (1). Four distinct mam-malian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as Beta-arrestin 1) and arrestin 3 (or Beta-arrestin 2) are ubiqui-tously expressed and bind to most GPCRs (2). Beta-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). Beta arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that Beta-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
Catalog #A3577-01H
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No10E5
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 0.02% sodium azide and 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Phe386 of human SGK2 protein.tissues using SGK2 antibody.
SpecificityRecongnizes endogenous levels of human total SGK2 protein. Species Crossreactivity: Rat, mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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