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Artemin, Recombinant, Human (GFRalpha3-RET Ligand)
Artemin, Recombinant, Human (GFRalpha3-RET Ligand)
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| Catalog # | A3580-52 | | Description | Recombinant Human Artemin is a disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2x113 amino acids. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gly-Gly-Pro-Gly. | | | | Dimers and Aggregates: | | 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Artemin is fully biologically active when compared to standard. The activity is calculated by it's ability to promote survival and neurite outgrowth and dorsal root ganglion neurons. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm | | 2. Analysis by RP-HPLC, using a calibrated solution of Artemin as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 24.2kD | | Source | Recombinant, Human (E. coli) | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder in 10mM sodium citrate, pH 4.5, 150mM sodium chloride. Reconstitute the lyophilized Human Artemin in sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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