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You are here:Home » Antibodies » Antibodies-Enzymes » Anti -ASAH2 (N-Acylsphingosine Amidohydrolase 2) (Biotin)

Anti -ASAH2 (N-Acylsphingosine Amidohydrolase 2) (Biotin)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Catalog #A3597-35
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse ASAH2. The detection limit for rmASAH2 is approximately 5ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 20% crossreactivity with rh ASAH2.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS, 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
PurityPurified by mouse ASAH2 affinity chromatography and then biotinylated.
ImmunogenNS0-derived rmASAH2 (aa34-756)
SpecificitySelected for use as a detection antibody in mouse ASAH2 Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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