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Anti -ASAHL (N-Acylsphingosine Amidohydrolase-like)
Anti -ASAHL (N-Acylsphingosine Amidohydrolase-like)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP |
|
| ASAHL (N-acylsphingosine-amidohydrolase-like; also acid ceramidase-like protein) is a 30kD member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359aa preproprecursor that contains a 28aa signal sequence, a 96aa prosegment, and a 30-31kD, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa324-359, while another has a two aa substitution for aa 198-359. The human proform (aa29-359) is 78% aa identical to mouse ASAHL proform. | | | Catalog # | A3597-42A | | Applications | Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | ELISA: 0.5-1.0ug/ml | | Western Blot: 1-2ug/ml | | Immunoprecipitation: 25ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9H66 | | Host | Mouse | | Source | Human | | Concentration | ~500ug/ml | | Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | CHO-derived, recombinant human ASAHL aa29-359; Q02083. | | Specificity | Recognizes human ASAHL. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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