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You are here:Home » Antibodies » Abs to Enzymes » Anti -ASAHL (N-Acylsphingosine Amidohydrolase-like)

Anti -ASAHL (N-Acylsphingosine Amidohydrolase-like)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
ASAHL (N-acylsphingosine-amidohydrolase-like; also acid ceramidase-like protein) is a 30kD member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359aa preproprecursor that contains a 28aa signal sequence, a 96aa prosegment, and a 30-31kD, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa324-359, while another has a two aa substitution for aa 198-359. The human proform (aa29-359) is 78% aa identical to mouse ASAHL proform.
Catalog #A3597-42A
ApplicationsSuitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H66
HostMouse
SourceHuman
Concentration~500ug/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenCHO-derived, recombinant human ASAHL aa29-359; Q02083.
SpecificityRecognizes human ASAHL.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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