Anti -ASC (Apoptosis-associated Speck-like Protein Containing a CARD, hASC, Caspase
Recruitment Domain-containing Protein 5, PYD and CARD Domain-containing Protein, Target of
Methylation-induced Silencing 1, PYCARD, CARD5, TMS1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IP IH |
|
| ASC [apoptosis-associated speck-like protein containing a CARD (caspase-recruitment domain)] is a bimodel protein containing a pyrin:paad domain (PYD/PAAD) and a caspase recruitment domain (CARD) (reviewed in McConnell and Vertino, 2004). ASC is also known as TMS1 (target of methylation-induced silencing). ASC was first identified in 1999 as a protein that forms cytoplasmic specks during retinoic acid induced differentiation or drug-induced apoptosis in HL60 cells. It was later independently identified in 2000 in a screen for targets of methylation-mediated silencing. In general proteins, like ASC/TMS1, containing PYD/PAAD and CARD domains play key roles in regulating apoptosis and inflammation signaling pathways. Mutations in a number of PYD/PAAD- and CARD-containing proteins have been linked to inflammatory diseases and cancer. There is evidence that ASC/TMS1 has roles in both apoptosis and inflammation signaling pathways. For example, ASC/TMS1 has been reported to function as a proapoptotic protein and has been shown to trigger apoptosis upon over expression in tumor cell lines. With respect to inflammation, ASC/TMS1 interacts with the CARD of procaspase-1 and induces aggregation of a protein complex called the inflammasome, thereby regulating caspase-1 activation and secretion of IL-1b. Additionally, ASC/TMS1 has been found to be subjected to methylation-mediated silencing in a significant proportion of human breast tumors and other cancers, including melanomas, glioblastomas, non-small lung cancers, gastric and colorectal cancers (reviewed in Parsons and Vertino, 2006). The loss of ASC/TMS1 expression in breast tumors and other cancers through methylation-mediated (epigenetic) silencing suggests that ASC/TMS1 has a role in tumorigenesis. In general, it is thought that methylation-mediated gene silencing contributes to tumorigenesis by inactivating genes involved in tumor suppression, and by conferring resistance to cell death signals by silencing genes that promote apoptosis. Thus methylation-mediated silencing of ASC/TMS1 may confer a survival advantage to tumor cells by enabling them to escape apoptosis. However, the precise role of ASC/TMS1 in the pathogenesis of cancer remains to be fully elucidated. Human ASC/TMS1 (isoform a) is a 195aa protein (~22kD), GenBank no. NP_037390.2. Three transcript variants encoding different isoforms have been identified for the ASC:TMS1 gene. ASC/TMS1 is normally highly expressed in immune cells, especially in neutrophils and cells of the macrophage:monocyte lineage; it is also expressed in many epithelial cell types (reviewed in Parsons and Vertino, 2006). | | | Catalog # | A3598-05L | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:2000 | | Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000 | | Immunoprecipitation: 1:50-1:200 | | Immunohistochemistry: Frozen | | Optimal dilutions to be determined by the researcher. | | Positive Control | Spleen, lymphoid, many cancer cell lines | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to aa83-102 (GQLQAATHQGSGAAPAGIQA) of human ASC/TMS1; GenBank no. NP_037390.2, which is also referred-as ASC/TMS1 isoform a. Isoform a is a 195 aaprotein. | | Specificity | Recognizes human ASC/TMS1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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