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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -ASC (Apoptosis-associated Speck-like Protein Containing a CARD, hASC, Caspase Recruitment Domain-containing Protein 5, PYD and CARD Domain-containing Protein, Target of Methylation-induced Silencing 1, PYCARD, CARD5, TMS1)

Anti -ASC (Apoptosis-associated Speck-like Protein Containing a CARD, hASC, Caspase
Recruitment Domain-containing Protein 5, PYD and CARD Domain-containing Protein, Target of
Methylation-induced Silencing 1, PYCARD, CARD5, TMS1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH
ASC [apoptosis-associated speck-like protein containing a CARD (caspase-recruitment domain)] is a bimodel protein containing a pyrin:paad domain (PYD/PAAD) and a caspase recruitment domain (CARD) (reviewed in McConnell and Vertino, 2004). ASC is also known as TMS1 (target of methylation-induced silencing). ASC was first identified in 1999 as a protein that forms cytoplasmic specks during retinoic acid induced differentiation or drug-induced apoptosis in HL60 cells. It was later independently identified in 2000 in a screen for targets of methylation-mediated silencing. In general proteins, like ASC/TMS1, containing PYD/PAAD and CARD domains play key roles in regulating apoptosis and inflammation signaling pathways. Mutations in a number of PYD/PAAD- and CARD-containing proteins have been linked to inflammatory diseases and cancer. There is evidence that ASC/TMS1 has roles in both apoptosis and inflammation signaling pathways. For example, ASC/TMS1 has been reported to function as a proapoptotic protein and has been shown to trigger apoptosis upon over expression in tumor cell lines. With respect to inflammation, ASC/TMS1 interacts with the CARD of procaspase-1 and induces aggregation of a protein complex called the inflammasome, thereby regulating caspase-1 activation and secretion of IL-1b. Additionally, ASC/TMS1 has been found to be subjected to methylation-mediated silencing in a significant proportion of human breast tumors and other cancers, including melanomas, glioblastomas, non-small lung cancers, gastric and colorectal cancers (reviewed in Parsons and Vertino, 2006). The loss of ASC/TMS1 expression in breast tumors and other cancers through methylation-mediated (epigenetic) silencing suggests that ASC/TMS1 has a role in tumorigenesis. In general, it is thought that methylation-mediated gene silencing contributes to tumorigenesis by inactivating genes involved in tumor suppression, and by conferring resistance to cell death signals by silencing genes that promote apoptosis. Thus methylation-mediated silencing of ASC/TMS1 may confer a survival advantage to tumor cells by enabling them to escape apoptosis. However, the precise role of ASC/TMS1 in the pathogenesis of cancer remains to be fully elucidated. Human ASC/TMS1 (isoform a) is a 195aa protein (~22kD), GenBank no. NP_037390.2. Three transcript variants encoding different isoforms have been identified for the ASC:TMS1 gene. ASC/TMS1 is normally highly expressed in immune cells, especially in neutrophils and cells of the macrophage:monocyte lineage; it is also expressed in many epithelial cell types (reviewed in Parsons and Vertino, 2006).
Catalog #A3598-05L
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000
Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000
Immunoprecipitation: 1:50-1:200
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive ControlSpleen, lymphoid, many cancer cell lines
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa83-102 (GQLQAATHQGSGAAPAGIQA) of human ASC/TMS1; GenBank no. NP_037390.2, which is also referred-as ASC/TMS1 isoform a. Isoform a is a 195 aaprotein.
SpecificityRecognizes human ASC/TMS1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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