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You are here:Home » Antibodies » Abs to Proteins » Anti -ASCL2 (Achaete-Scute Complex Homolog 2 (Drosophila), Achaete-scute Homolog 2, ASH2, ASH-2, bHLHa45, BHLHA45, Class A Basic Helix-loop-helix Protein 45, hASH2, HASH2, Mash2, MASH2)

Anti -ASCL2 (Achaete-Scute Complex Homolog 2 (Drosophila), Achaete-scute Homolog 2, ASH2,
ASH-2, bHLHa45, BHLHA45, Class A Basic Helix-loop-helix Protein 45, hASH2, HASH2, Mash2,
MASH2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IH
ASCL2 is a class II member of the HLH family of transcription factors. Its predicted MW is 20kD. ASCL2 shows restricted expression, being limited to intestinal Lgr5+ stem cells and first trimester placental cytotrophoblasts. In the intestine, ASCL2 is under the control of Wnts and serves to maintain an epithelium stem cell pool. In the placenta, ASCL2 acts to maintain the pool of cytotrophoblasts at the expense of synctiotrophoblasts, thus promoting placental growth. Human ASCL2 is 193aa in length. It contains one poly-Arg motif (aa36-39), a DNA binding sequence (aa53-63) and an HLH domain (aa64-103). ASCL2 forms heterodimers with daughterless homologs (E22, E2A and HEB). Over aa1-49, human ASCL2 shares 59aa identity with mouse Mash2.
Catalog #A3619-81B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 200ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa1-49 from human ASCL2/Mash2 expressed in E. coli (Q99929).
SpecificityRecognizes human ASCL2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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