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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -Ascorbate Oxidase (AO, Ascorbase, ASO, L-ascorbate Oxidase Precursor)

Anti -Ascorbate Oxidase (AO, Ascorbase, ASO, L-ascorbate Oxidase Precursor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP
Ascorbate Oxidase is a copper-containing enzyme. It catalyzes the following reaction:
Ascorbic acid + O2 -----> Dehydroascorbic acid + H2O.
Ascorbate Oxidase is widely distributed and is only present in plants. Its biological role in plants is still not clear. Studies after increasing and reducing the enzyme activity suggest that it has a positive effect on plant growth. In addition, it has been proposed that the enzyme may be involved in plant defense by modifying the ascorbate level and thus redox state in apoplast.
Catalog #A3700-01
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:25,000-1:125,000
Western Blot:1:2000-1:10,000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O.
PurityPurified by Ion Exchange chromatography.
ImmunogenAscorbate Oxidase (cucurbita).
SpecificityRecognizes Ascorbate Oxidase from Cucurbita. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Ascorbate Oxidase (cucurbita).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAscorbase, ASO, L-ascorbate oxidase precursor


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