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Specifications

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples. The Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at lambda = 570nm) or fluorometric (Ex/Em = 535/587nm) methods. The assay can detect 0.01-10nmol of ascorbic acid per assay in various samples.
Catalog #	A3774-60
Kit Components	A3774-60A: Assay Buffer, 1x25ml.
	A3774-60B: Probe, 1x200ul.
	A3774-60C: Catalyst, 1x500ul.
	A3774-60D: Enzyme Mix, 1x1vial.
	A3774-60E: Standard, 1x1vial (20umole).
Storage and Stability	Store all components at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.