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Anti -ASH1 (MASH1, Helix-loop-helix Transcription Factor, Achaete-scute Complex Homolog-like1, ASCL1)
Anti -ASH1 (MASH1, Helix-loop-helix Transcription Factor, Achaete-scute Complex
Homolog-like1, ASCL1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E B IC |
|
| ASH1 is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. | | | Catalog # | A3782-02A | | Applications | Suiutable for use in Immunocytochemistry, Western Blot and Direct ELISA. Other applications have not been tested. | | Recommended Dilutions | Immunocytochemistry: 8-25ug/ml. Immersion fixed NTera-2 human testicular embryonic carcinoma cell line treated with retinoic acid | | Western Blot: 1ug/ml with recombinant mouse ASCL1 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 8B1 | | Host | Rat | | Source | Mouse | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant mouse ASH1 corresponding to aa74-173 expressed in E. coli. (Accession #Q53WV9) | | Specificity | Recognizes mouse ASH1. Species Sequence Homology: human | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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