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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -ASH1 (MASH1, Helix-loop-helix Transcription Factor, Achaete-scute Complex Homolog-like1, ASCL1)

Anti -ASH1 (MASH1, Helix-loop-helix Transcription Factor, Achaete-scute Complex
Homolog-like1, ASCL1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IC
ASH1 is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation.
Catalog #A3782-02A
ApplicationsSuiutable for use in Immunocytochemistry, Western Blot and Direct ELISA. Other applications have not been tested.
Recommended DilutionsImmunocytochemistry: 8-25ug/ml. Immersion fixed NTera-2 human testicular embryonic carcinoma cell line treated with retinoic acid
Western Blot: 1ug/ml with recombinant mouse ASCL1
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8B1
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to aa74-173 (Gln160Glu) from mouse ASH1 expressed in E. coli. (Accession #Q53WV9)
SpecificityRecognizes mouse ASH1. Species Sequence Homology: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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