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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -ASH2 (ASH2L1, ASH2L2, Absent, Small, Homeotic-Like 2, Trithorax protein ASH2)

Anti -ASH2 (ASH2L1, ASH2L2, Absent, Small, Homeotic-Like 2, Trithorax protein ASH2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP IF
The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 on Lys4 and functions as a transcriptional co-ac-tivator (1). While yeast contain only one known Set1 protein,
mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3 and MLL4, all of which methylate his-tone H3 on Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common structural subunits WDR5, RBBP5 and ASH2L (2-6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies,
suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6).
Catalog #A3782-10F
ApplicationsSuitable for use in RIA, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:1000
Western Blot: 1:2000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No10E6
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptide derived from the sequence of human ASH2L protein (KLH).
SpecificityRecongnizes endogenous levels of human all known isoforms of the ASH2L protein. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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