Anti -ASH2 (ASH2L1, ASH2L2, Absent, Small, Homeotic-Like 2, Trithorax protein ASH2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Purified |
B IP IF |
|
| The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 on Lys4 and functions as a transcriptional co-ac-tivator (1). While yeast contain only one known Set1 protein, | | | mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3 and MLL4, all of which methylate his-tone H3 on Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common structural subunits WDR5, RBBP5 and ASH2L (2-6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, | | | suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6). | | | Catalog # | A3782-10F | | Applications | Suitable for use in RIA, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:1000 | | Western Blot: 1:2000 | | Immunoprecipitation: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Clone No | 10E6 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide. | | Purity | Purified | | Immunogen | Synthetic peptide derived from the sequence of human ASH2L protein (KLH). | | Specificity | Recongnizes endogenous levels of human all known isoforms of the ASH2L protein. Species crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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