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You are here:Home » Molecular Biology » MB-Protein Kinases » ASK1, active, Recombinant, Human (Apoptosis Signal-regulating Kinase 1, ASK-1, Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase 5, MEK Kinase 5, MEKK 5, MEKK5)

ASK1, active, Recombinant, Human (Apoptosis Signal-regulating Kinase 1, ASK-1,
Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase
5, MEK Kinase 5, MEKK 5, MEKK5)

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Specifications

ASK1, also known as MEKK5 or MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Over-expression of ASK1 induces apoptotic cell death and is activated in cells treated with tumor necrosis factor alpha (TNFa) (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
Source:
Recombinant human ASK1 (amino acids 649-946) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_005923.
Catalog #A3794-02
ActivityThe specific activity of ASK1 was determined to be 97 nmol/min/mg using a myelin basic protein (MBP) substrate (see activity assay protocol).
Storage and StabilityAliquot to avoid repeated freezing and thawing, and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Weight60kD
SourceBaculovirus in Sf9 insect cells
Purity90% by densitometry
ConcentrationAs reported
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=2.7.11.25


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