Anti -ASK1, phosphorylated (Ser83) (Apoptosis Signal-regulating Kinase 1, ASK-1,
Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase
5, MEK Kinase 5, MEKK 5, MEKK5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| Apoptosis signal-regulating kinase 1 (ASK1), a MAP kinase kinase kinase, plays essential roles in stress-induced apoptosis (1,2). ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38 (3). Overexpression of ASK1 activates JNK and p38 and induces apoptosis through signals involving the mitochondrial cell death pathway in several cell types. Embryonic fibroblasts or primary neurons derived from ASK1-/-mice are resistant to various stress-induced JNK and p38 activation and cell death (4,5). ASK1 is a substrate for phosphorylation at Ser83 by Akt and this phosphorylation is associated with a decrease in stimulated ASK1 kinase activity (6). | | | Catalog # | A3794-15 | | Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunoprecipitation: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. | | Purity | Purified by protein A and immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to residues around Ser83 of human ASK1 (KLH coupled). | | Specificity | Detects overexpressed human ASK1 only when phosphorylated at Ser83. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | EC=2.7.11.25 |
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