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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -ASK1, phosphorylated (Ser83) (Apoptosis Signal-regulating Kinase 1, ASK-1, Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase 5, MEK Kinase 5, MEKK 5, MEKK5)

Anti -ASK1, phosphorylated (Ser83) (Apoptosis Signal-regulating Kinase 1, ASK-1,
Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase
5, MEK Kinase 5, MEKK 5, MEKK5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B
ASK-1 (apoptosis signal-regulating kinase 1-also referred to as MEK Kinase-5 or MAPKKK5) is
a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The
overall amino acid sequence identity between the mouse and human ASK1 is 91.9%. ASK-1 interacts with CDKN1A known as p21, WAF1, CIP1).
Catalog #A3794-16
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:10,000
Western Blot: 1:1000 155 kD
Optimal dilutions to be determined by the researcher.
positive control SW1353 whole cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
PurityPurified by delipidation, salt fractionation and ion exchange chromatography
ImmunogenA synthetic peptide corresponding to amino acids 76-87 of human ASK-1 protein (KLH).
SpecificityRecognizes human ASK1 when phosphorylated at SEr83. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=2.7.11.25


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