Anti -ASK1, phosphorylated (Ser83) (Apoptosis Signal-regulating Kinase 1, ASK-1,
Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase
5, MEK Kinase 5, MEKK 5, MEKK5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Highly Purified |
E B |
|
| ASK-1 (apoptosis signal-regulating kinase 1-also referred to as MEK Kinase-5 or MAPKKK5) is | | | a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The | | | overall amino acid sequence identity between the mouse and human ASK1 is 91.9%. ASK-1 interacts with CDKN1A known as p21, WAF1, CIP1). | | | Catalog # | A3794-16 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:5000-1:10,000 | | Western Blot: 1:1000 155 kD | | Optimal dilutions to be determined by the researcher. | | positive control | SW1353 whole cell lysates | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. | | Purity | Purified by delipidation, salt fractionation and ion exchange chromatography | | Immunogen | A synthetic peptide corresponding to amino acids 76-87 of human ASK-1 protein (KLH). | | Specificity | Recognizes human ASK1 when phosphorylated at SEr83. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | EC=2.7.11.25 |
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