| Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. |
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| Catalog # | A3879-15 |
| It catalyzes the reaction | Aspartate + alpha-Ketoglutarate Oxaloacetate + Glutamate |
| Diagnostically, it is almost always measured in units/liter (U/L). In this AST Assay Kit, an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (lambda max = 450nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of AST with a detection limit of 10mU per well. |
| Kit Components | • AST *Assay Buffer, 1x25ml |
| • AST Enzyme Mix, 1x1 vial |
| • Developer, 1x1 vial |
| • AST Substrate, 1x1 vial |
| • Glutamate Standard (0.1M), 1x0.1ml |
| • AST Positive Control, 1x1 vial |
| Storage and Stability | Store kit at -20°C, protect from light. Allow *Assay Buffer to warm to room temperature before use. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
