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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Aspartate BioAssay™ Kit

Aspartate BioAssay™ Kit

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Specifications

L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino A3880-01acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle, participates in gluconeogenesis and transports reducing equivalents between the cytosol and the mitochondria via the malate-aspartate shuttle. Aspartate also stimulates NMDA receptors (not as strongly as glutamate) and hence serves as an excitatory neurotransmitter in the brain and is an excitotoxin.
Catalog #A3881
The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570nm) and fluorescent (Ex/Em 535/587nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10nmoles/well (2-200uM).
Kit componentsAspartate Assay Buffer, 1x25ml
Probe (DMSO solution), 1x200ul
Serum CleanUp Mix, 1x25ml
Aspartate Enzyme Mix, 1x1vial
Aspartate Substrate Mix, 1x1vial
Conversion Mix, 1x1vial
Aspartate Standard (100 mM),1x100ul
Storage and StabilityStore at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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