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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH

A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. A3883-01C has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. A3880-01C has also been used for the identification of aspergillosis in human tissue sections.
Catalog #	A3883-01C
Applications	Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Paraffin): 1:300
	Requires protein digestion pretreatment of paraffin sections.
	Western Blot: Detects a band of ~106kD of Aspergillus fumigatus wall fraction (WF).
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c ABom mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	10B218
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Wall fraction (WF) of Aspergillus fumigatus.
Specificity	Recognizes members of the Aspergillus spp including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae. Does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.