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You are here:Home » Antibodies » Abs to Proteins » Anti -ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, FLJ10517, FLJ10549, MCPH5)

Anti -ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary
Autosomal Recessive 5, HGNC:19048, FLJ10517, FLJ10549, MCPH5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH
ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.
Catalog #A3883-40A
Molecular Weight218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol. 14, No. 15 2155-2165.
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: Tested in direct ELISA against the ASPM immunogen.
Immunohistochemistry (Paraffin): 1:300. Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman ductal carcinoma tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenLinear peptide corresponding to human ASPM conjugated to KLH. (NP_060606). Epitope
SpecificityRecognizes human ASPM. Species Crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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