| Catalog # | A3935-50C |
| Applications | Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunofluorescence: 1:100-1:3000 |
| Western Blot: 1:100-1:3000 |
| Immunohistochemistry: 1:100-1:3000 |
| Optimal dilutions to be determined by the researcher. |
| Cellular Localization | Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. |
| Note: Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. Belongs to the ATG9 family. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to the C-terminal of human APG9A (APG9L1). The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). |
| Specificity | IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Species Crossreactivity: Human, rat. Other species not yet tested. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | Autophagy-related Protein 9A, APG9-like 1, Cytoplasm to Vacuole Targeting Protein 7, Autophagy-related Protein 9, APG9A |
