| ATH 1 (MATH 1, NeuroD Family) belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription. |
|
| Catalog # | A3950-01 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot (ECL): 1:200-1:1000 |
| Immunohistochemistry: 1:200-1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | A3950-01A |
| IMMUNOHISTOCHEMISTRY Protocol | 1. Dissect tissues and freeze on dry ice. |
| 2. Cut on a cryostat -10um slices. |
| 3. Dry slides at RT for 30-90 min. |
| 4. Fix with cold Acetone/Methanol (50/50) for 2 min. |
| Either process further or store at -20°C. Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation for 10 minutes at 37°C, followed by 3 washes with PBS). |
| 5. Let air dry. |
| 6. Treat with PBS for 5 min. |
| 7. Incubate in 50mM ammonium chloride for 30 min. |
| 8. Treat with PBS for 5 min. |
| 9. Treat with Blocking serum for 30-45 min. |
| 10. Treat with A3950-01 for 90 min. |
| 11. Wash 3 times for 5 min. |
| 12. Secondary fluorescent conjugate antibody for 30 min. |
| 13. Wash 3 times for 5 min and mount. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2. No preservative added. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa188-200, SFNNDKKLSKYET of human ATH1. |
| Specificity | Recognizes human ATH 1 at ~38kD. Species Crossreactivity: rodent. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
