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You are here:Home » Antibodies » Antibodies-Transcription Factors » Anti -ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor)

Anti -ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
ATH 1 (MATH 1, NeuroD Family) belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription.
Catalog #A3950-01
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot (ECL): 1:200-1:1000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide A3950-01A
IMMUNOHISTOCHEMISTRY Protocol1. Dissect tissues and freeze on dry ice.
2. Cut on a cryostat -10um slices.
3. Dry slides at RT for 30-90 min.
4. Fix with cold Acetone/Methanol (50/50) for 2 min.
Either process further or store at -20°C. Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation for 10 minutes at 37°C, followed by 3 washes with PBS).
5. Let air dry.
6. Treat with PBS for 5 min.
7. Incubate in 50mM ammonium chloride for 30 min.
8. Treat with PBS for 5 min.
9. Treat with Blocking serum for 30-45 min.
10. Treat with A3950-01 for 90 min.
11. Wash 3 times for 5 min.
12. Secondary fluorescent conjugate antibody for 30 min.
13. Wash 3 times for 5 min and mount.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa188-200, SFNNDKKLSKYET of human ATH1.
SpecificityRecognizes human ATH 1 at ~38kD. Species Crossreactivity: rodent.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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