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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor)

Anti -ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
ATH 1 (MATH 1, NeuroD Family) belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription.
Catalog #A3950-01
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot (ECL): 1:200-1:1000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide A3950-01A
IMMUNOHISTOCHEMISTRY Protocol1. Dissect tissues and freeze on dry ice.
2. Cut on a cryostat -10um slices.
3. Dry slides at RT for 30-90 min.
4. Fix with cold Acetone/Methanol (50/50) for 2 min.
Either process further or store at -20°C. Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation for 10 minutes at 37°C, followed by 3 washes with PBS).
5. Let air dry.
6. Treat with PBS for 5 min.
7. Incubate in 50mM ammonium chloride for 30 min.
8. Treat with PBS for 5 min.
9. Treat with Blocking serum for 30-45 min.
10. Treat with A3950-01 for 90 min.
11. Wash 3 times for 5 min.
12. Secondary fluorescent conjugate antibody for 30 min.
13. Wash 3 times for 5 min and mount.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa188-200, SFNNDKKLSKYET of human ATH1.
SpecificityRecognizes human ATH 1 at ~38kD. Species Crossreactivity: rodent.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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