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You are here:Home » Antibodies » Abs to Enzymes, Synthase » Anti -ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB)

Anti -ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH FC
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.
Catalog #A3999-67
ApplicationsSuitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:1000
Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a portion of amino acids within aa142-171 from the center region of human ATP5B conjugated to KLH.
SpecificityRecognizes human ATP5B.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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