| ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria. Mutations in the ATP7b gene can cause Wilson’s disease, an inherited disorder causing copper poisoning in the brain and liver. |
|
| Catalog # | A3999-82A |
| Applications | Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (Paraffin): 1:500 |
| Western Blot: 1:1000. Band at ~165kD |
| Immunocytochemistry: 1:500 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | HuH7 hepatocarcinoma cell extracts |
| Cellular Localization | Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140kD is located in mitochondria. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in Tris citrate/phosphate buffer, pH 7.5, 0.1% sodium azide. |
| Purity | Purified by affinity chromatography |
| Immunogen | Synthetic peptide made to an internal sequence near the N-terminus of human ATP7b |
| Specificity | Recognizes human and mouse ATP7b. This antibody has specifically been tested on mouse brain and liver. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | ATPase, Copper Transporting, beta Polypeptide |
