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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -ATP7b (PWD, WC1, WND)

Anti -ATP7b (PWD, WC1, WND)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria. Mutations in the ATP7b gene can cause Wilsonís disease, an inherited disorder causing copper poisoning in the brain and liver.
Catalog #A3999-82A
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin): 1:500
Western Blot: 1:1000. Band at ~165kD
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.
Positive ControlHuH7 hepatocarcinoma cell extracts
Cellular LocalizationGolgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140kD is located in mitochondria.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris citrate/phosphate buffer, pH 7.5, 0.1% sodium azide.
PurityPurified by affinity chromatography
ImmunogenSynthetic peptide made to an internal sequence near the N-terminus of human ATP7b
SpecificityRecognizes human and mouse ATP7b. This antibody has specifically been tested on mouse brain and liver.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesATPase, Copper Transporting, beta Polypeptide


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