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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified B IC

ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria. Mutations in the ATP7b gene can cause Wilson’s disease, an inherited disorder causing copper poisoning in the brain and liver.
Catalog #	A3999-82B
Applications	Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000. Recognizes a band at 165kD, representing ATP7b protein. Also recognizes a band at ~220kD.
	Immunocytochemistry: 1:500 using ovarian carcinoma cells
	Optimal dilutions to be determined by the researcher.
Positive Control	HuH7 hepatocarcinoma cell extracts
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid containing 0.1% sodium azide
Purity	Purified
Immunogen	Synthetic peptide made to an internal sequence nearer the C-terminus of human ATP7b
Specificity	Recognizes human ATPb7. Results on mouse appear to be negative.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	ATPase, Copper Transporting, beta Polypeptide