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You are here:Home » Antibodies » Antibodies-Enzymes, ATPase » Anti -ATPase, Ca (Plasma Membrane, PMCA)

Anti -ATPase, Ca (Plasma Membrane, PMCA)


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Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH IC
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuous removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX) in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+ and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
Catalog #A3999-99H
ApplicationsSuitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen, paraffin): 1:500
Western Blot: 1:1000. Detects a band of ~140kD. Also detects bands at ~95kD and ~180kD which may represent aggregation and/or natural proteolytic products of PMCA ATPase.
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No6D131
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
ImmunogenPurified human erythrocyte PMCA ATPase (plasma membrane calcium pump ATPase).
SpecificityRecognizes all known (currently four) isoforms of human plasma membrane PMCA ATPase. Recognizes an epitope between aa724-783 of human APTase, Ca. Species Crossreactivity: rat, mouse, sheep, canine, monkey, rabbit, feline, chicken, amphibian
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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