Anti -ATM (Ataxia Telangiectasia Mutated, ATA, AT Complementation Group A, ATC, ATD, ATE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IH |
|
| Ataxia-telangiectasia (A-T) is a recessive childhood disease caused by mutations in the ATM (AT-mutated) gene. Symptoms include neurological abnormalities that cause unsteady posture, dilated blood vessels, infertility, radiation sensitivity, immune deficiencies and lymphoid malignancies. It appears that the diverse defects seen in ATM null mammals are manifestations of disparate signal transduction defects. The ATM protein is related to a family of proteins through a c-terminal phoshatidylinositol 3-kinase (PI3-kinase) domain. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM activates p53, increasing the p21Cip1 levels, thus blocking activation of cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates cdc25C, preventing it from phosphorylating the inhibitory phosphotyrosine residue on cdc2/cdk1, thus preventiing the G2/M transition. | | | Catalog # | A4000-25E | | Applications | Suitable for use in Western Blot and Immunohistochemistry (paraffin). Not suitable for use in Immunohistochemistry on frozen sections or in Immunoprecipitation. Other applications not tested. | | Recommended Secondary Antibodies | I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb | | Recommended Dilutions | Western Blot: 1:250-1:500 | | Immunohitochemistry (Paraffin): 1:25 | | Paraffin sections require antigen retrieval using heat treatment prior to staining. Two hour incubation with the antibody is recommended. | | Optimal dilutions to be determined by researcher | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in PBS, 0.1% sodium azide, before the addition of glycerol to 40%. | | Purity | Serum | | Immunogen | Recombinant human ATM fragment (aa1980-2338). | | Specificity | Recognizes the human ATM protein, a 350kD polypeptide that is the product of the ATM gene. Species Crossreactivity: Monkey, mouse and hamster. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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