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You are here:Home » Molecular Biology » MB-Enzymes, ATPase » ATPase, Ca, Autoinhibitory Domain, Human (c28r2) Control Peptide

ATPase, Ca, Autoinhibitory Domain, Human (c28r2) Control Peptide

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Specifications

The 28aa Peptide sequence (~CT, cytoplasmic) corresponding to human Ca+-ATPases, autoinhibitory domain
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide; RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump.
Catalog #A4000-49C
Ca+-pump is a Mg+-dependent enzyme that catalyzes the hydrolysis of ATP with the transport of Ca+. Plasma membrane Ca+-ATPase (calcium pump) exists in several isoforms (human isofroms 1, 1084 aa; 1b, 1220 aa; from 2, 198/1243 aa; 3a/b 1173/1220 aa; 4, 1205 aa) and numerous sub-isoforms have been identified in various species. Ca+-pump displays 10 TM domains, with cytoplasmic N and C-termini. C28R2 is located in within the cytoplasmic, C-terminal domain of Ca+-ATPases. The C28R2 sequence is very conserved in various isoforms among various species.
SourceHuman synthetic peptide
PurityHighly purified
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
SpecificityThe 28 AA human sequence is 100% conserved in human Ca+-ATPase isoforms 1, 2, 3b, 96% in 1b, 92% in 4 and 85% in 3a/3b. It is also 100% conserved in mouse and rat isoform 2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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