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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase, Ca (ATP2B1, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium ATPase 1, PMCA1 ATPase)

Anti -ATPase, Ca (ATP2B1, Plasma membrane calcium ATPase isoform 1, plasma membrane
calcium ATPase 1, PMCA1 ATPase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium
from the cytosol to the extracellular space. This membrane-bound enzyme is related to
a number of other ATPases including the SERCA ATPase and the sodium/potassium
pump. There are four different genes encoding PMCA ATPase and studies have revealed 20
isoforms of the pump generated by alternate splicing of the primary gene products.
mRNA distribution studies show that gene products 1 and 4 are transcribed in most
tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing
variants has also been found to be tissue specific. In the pancreas, where insulin
secretion is calcium dependent, the beta cells only express the 4b isoform, however the
alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that
different splice variants have different affinities for calcium and calmodulin.
Catalog #A4000-50A2
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Detects ~134kD and ~130kD proteins representing PMCA 1a and 1b ATPase from rat samples
Optimal dilutions to be determined by the researcher.
Localization Cell Membrane; Multi-pass membrane protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS containing 1mg/ml BSA and 0.05% sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide (ANNSVAYSGVKNSIKEAN) corresponding to amino acid residues 5-22 from rat PMCA1 ATPase protein. This sequence is completely conserved between rat and human PMCA1 ATPase.
SpecificityRecognizes rat and human PMCA 1a and 1b ATPase
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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