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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase, Ca (Plasma Membrane 1, PMCA1)

Anti -ATPase, Ca (Plasma Membrane 1, PMCA1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #A4000-50A6
The Ca(2+)-ATPases are a family of plasma membrane pumps encoded by at least 4 genes ATP2B1 on chromosome 12q21; ATP2B2; ATP2B3 on Xq28; and ATP2B4 on 1q25. The proteins share 84% aa sequence identity and 76% nucleic acid sequence homology. The function of calcium-transporting ATPase found in different membranes is to lower cytoplasmic Ca(2+) concentration by pumping Ca(2+) to luminal or extracellular spaces. Mutations in a plasma membrane Ca(2+)-ATPase gene cause deafness in deafwaddler mice.
ApplicationSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 4ug/ml
Immunohistochemistry (paraffin): 8ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10K342
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman erythrocyte Ca2+-ATPase.
SpecificityRecognizes human PMCA. Species Crossreactivity: mouse, rat, chicken, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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