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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase, Ca (ATP2B2, PMCA2 ATPase, Plasma membrane calcium ATPase isoform 2, plasma membrane calcium ATPase 2)

Anti -ATPase, Ca (ATP2B2, PMCA2 ATPase, Plasma membrane calcium ATPase isoform 2, plasma
membrane calcium ATPase 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
Catalog #A4000-50B3
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000. Detects ~127kD and ~133kD proteins representing PMCA 2a and 2b ATPase from rat brain microsomal fractions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa5-19, TNSDFYSKNQRNESS, of human PMCA2 ATPase protein. This sequence is completely conserved between rat and human PMCA2 ATPase.
SpecificityRecognizes human PMCA 2a and 2b ATPase.
Species CrossreactivityRat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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