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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase PMCA4

Anti -ATPase PMCA4

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Plasma membrane calcium-ATPase 4 (PMCA4) expression shows little variation across eight drug-treated cell lines and was found to be superior to GAPDH and HPRT1, commonly used reference genes. PMCA4 can be used as a reference gene for normalizing ABC transporter expression in a drug-resistant lung carcinoma cell line. PMCA4 is a good housekeeping gene for normalization of gene expression for polytopic membrane proteins including transporters and receptors.
Catalog #A4000-50D
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:15,000
Western Blot: 1:1000
Immunohistochemistry (Frozen & Paraffin): 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J323
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified
ImmunogenPurified human erythrocyte PMCA ATPase. Epitope: aa51-75. This sequence is common to both PMCA4a and 4b ATPase splice variants.
SpecificityRecognizes human PMCA4 4 isoforms, 4a and 4b, at ~133 and 129kD, respectively. Species Crossreactivity: porcine and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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