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You are here:Home » Molecular Biology » MB-Enzymes, ATPase » ATPase, Ca, Rat, Control Peptide (Plasma Membrane, PMCA)

ATPase, Ca, Rat, Control Peptide (Plasma Membrane, PMCA)


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A 20aa Peptide sequence near the N-terminus of mature Rat NCKX2 (1) was selected for antibody production.
Catalog #A4000-50H
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers- Three NCX (NCX1, NCX2, and NCX3) and two in the NCKX family (NCKX1 and NCKX2). NCX shares significant sequence homology (~70%), displays 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.
NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide; RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump.
ApplicationsELISA: 1:10-50K for neat serum and 0.5-1ug/ml for affinity purified.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRat synthetic peptide
PurityChromatographically purified
FormSupplied as a liquid in PBS, pH 7.2.
SpecificityThe 20 AA human sequence is 75-100% conserved in mouse Ca+-ATPase isoforms 1, 1b, 2, 3a/b and 4 in mouse, rat and human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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