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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATPase alpha 1, Na, K

Anti -ATPase alpha 1, Na, K

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
Catalog #A4000-52
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.05-0.1ug/ml detected the a-1 subunit in 20ug of rat brain microsomal preparations using chemiluminescence. To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Immunohistochemistry: 1-10ug/ml on ice-cold methanol-fixed tissue.
Immunoprecipitation: 2ug immunoprecipitated the a-1 subunit from rat brain microsomal preparations.
Optimal dilutions to be determined by the researcher.
SourceCulture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.T.1
HostMouse
SourceRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
SpecificityRecognizes rabbit Na+/K+ ATPase alpha-1 subunit. MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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